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NFPA SUPPLIER Santa Cruz Biotechnology, Inc. 2145 Delaware Avenue Santa Cruz Ethidium bromide is a frameshift mutagen (causes changes to DNA) and intercalating agent (acts like insertion mutations). Although evidence shows that when applied in vitro it is a mutagen, contact with mammalian cells in other circumstance requires higher concentrations than that used in labs to cause an immediate risk to humans. In this article the mutagenic properties of the Diamond™ Nucleic Acid Dye and ethidium bromide are compared using the Ames test. Ethidium bromide is mutagenic and moderately toxic and must be handled with care. The powder form is considered an irritant to the upper respiratory tract, eyes, and skin. This is unlike ethidium bromide, which is genotoxic at typical usage concentrations (0.5 µg/ml). Both dyes are cytotoxic, however, at commercially available stock concentrations.

P280 Wear protective gloves/ protective clothing/ eye protection/ face Page 1 of 6 Ethidium Bromide Solution MATERIAL SAFETY DATA SHEET SDS/MSDS This reagent is ideal for the environment requiring a safe , non-hazardous alternative to Ethidium Bromide. Novel Juice non toxic DNA Stain keeps your lab safe. Safe – Absence of mutagenity and low toxicity (LC>5000mg/kg) as compared to Ethidium Bromide. Low Environmental Impact – Compliance with the Clean Water Act standards. Ethidium bromide has been commonly used as a DNA stain for many years. However, ethidium bromide is harmful if swallowed, and is very toxic if inhaled.

ethidium bromide - Safety Data Sheet

Ethidium bromide is toxic. Wear gloves and a face mask when working with ethidium bromide in powdered form.

Functional Materials and Biomaterials - PDF Free Download

this is because it is impermeable to intact cell membranes.

Ethidium bromide. FAPyG. 2,6-diamino-4-hydroxy-5- with toxic substances or irradiation, but they also showed that DNA damage. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).Buffertar håller GelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) Cytotoxicity was accessed by ethidium bromide staining, MTT reduction, and TUNEL assays.
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paper on SYBR®Safe toxicity here. Ethidium bromide is highly toxic through inhalation. If dust or aerosol formation is possible, perform procedures in a chemical fume hood. When possible, purchase ethidium bromide tablets or ready-made stock solutions instead of powder. Containers should be kept closed when not in use.

Acetic acid (TAE buffer). CAS: 64-19-7. TAE buffer is slightly EtBr.
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In powder form, it is considered an irritant to the eyes and upper respiratory tract. Ethidium bromide has been shown to be mutagenic in various in vitro assays and is an aquatic toxin. Green 2008-03-03 GEL RED – A SAFER ALTERNATIVE TO TOXIC ETHIDIUM BROMIDE IN DNA RESEARCH Gel Red is an innovative, stable, non-hazardous, and environmentally safe fluorescent nucleic acid dye designed by Biotium as an alternative to toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA and RNA in agarose gels and polyacrylamide gels. Ethidium bromide induces mutations in cells and that is a fact.

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A natural DNA-intercalator plant benzo-c-phenanthridine alkaloid sanguinarine is more toxic for mouse transformed fibroblast L-cells in culture than synthetic DNA-intercalator ethidium bromide (EtB) and alkaloid berberine. Dimidium bromide is also an inhibitor of the L-cell growth.